Experimental Skills
Other Skills
Education
Molecular Biology (14yrs)
Nucleic acid extraction (DNA/RNA) and quantification
Gel Electrophoresis
PCR, PCR primer designing
Genotyping-RFLP
Functional studies- knockdown of genes using shRNA and siRNA
Single cell cloning
Sanger sequencing
Cell Culture (14yrs)
Stem cell culture
Neuronal induction of Stem cells
Mammalian cell lines (normal and cancer)
Primary tissue culture
Hybridoma technology
Fluorescent microscopy
Cell viability assays
Drug dosage formulations
Cytogenetics (14yrs)
Lymphocyte culturing – Chromosomal isolation
Karyotyping
Microscopy
FISH
Biochemical/Immunology assays (14yrs)
Protein and Antibody purification using Column and Affinity Chromatography
SDS-PAGE
ELISA
Western Blotting
Micro-array
TRAP assay
Flow cytometry
Gene expression assays (11yrs)
Reverse transcription
cDNA library preparation
TaqMan/SYBR green real Time PCR(qPCR)
RNAseq
Paired end RNAseq
Epigenetic techniques (11yrs)
Bisulphite modification
qualitative and quantitative Methylation specific PCR (MSP)
Bisulphite sequencing
Bioinformatics (12yrs)
Basic R programming Working proficiency on following bioinformatics analysis platforms: Gene expression, Epigenetic, Cancer databases, Transcriptomics, Alternative Splicing
Program management (10yrs)
Managed projects and teams using TRELLO, SLACK, ASANA)
Statistical Tools (10yrs)
SPSS & MedCalc
Others
Working proficiency in Microsoft Office, reference managers, WordPress
| 2012 – 2017 | Doctorate (Ph.D.) in Human Genetics (specialization Neuro-oncology) National Institute for Mental Health and Neurosciences |
| 2008 – 2010 | Master of Science (M.Sc.) in Biomedical Genetics Vellore Institute of Technology (VIT) University, Vellore, India |
| 2005 – 2008 | Bachelor of Science (B.Sc.) in Chemistry, Botany, Biotechnology St. Joseph’s College (Autonomous), Bangalore, India |