Skills revamped

EDUCATION

2005 – 2008

Bachelor of Science (B.Sc.)
Chemistry, Botany,
Biotechnology
St. Joseph’s College (Autonomous) Bengaluru, India

2008 – 2010

Master of Science (M.Sc.) 
Biomedical Genetics
Vellore Institute of Technology (VIT) University
Vellore, India

2012 – 2017

Doctorate (Ph.D.)
Human Genetics (specialization Neuro-oncology)
National Institute for Mental Health and Neurosciences
Bangalore, India

EXPERIMENTAL SKILLS

Molecular Biology (14yrs)

  • Nucleic acid extraction (DNA/RNA) and quantification
  • Gel Electrophoresis
  • PCR, PCR primer designing
  • Genotyping-RFLP
  • Functional studies- knockdown of genes using shRNA and siRNA
  • Single cell cloning
  • Sanger sequencing

Cell Culture (14yrs)

  • Stem cell culture
  • Neuronal induction of Stem cells
  • Mammalian cell lines (normal and cancer)
  • Primary tissue culture
  • Hybridoma technology
  • Fluorescent microscopy
  • Cell viability assays
  • Drug dosage formulations

Cytogenetics (14yrs)

  • Lymphocyte culturing – Chromosomal isolation
  • Karyotyping
  • Microscopy
  • FISH

Biochemical/Immunology assays (14yrs)

  • Protein and Antibody purification using Column and Affinity Chromatography
  • SDS-PAGE
  • ELISA
  • Western Blotting
  • Micro-array
  • TRAP assay
  • Flow cytometry

Gene expression assays (11yrs)

  • Reverse transcription
  • cDNA library preparation
  • TaqMan/SYBR green real Time PCR(qPCR)
  • RNAseq
  • Paired end RNAseq

Epigenetic techniques (11yrs)

  • Bisulphite modification
    qualitative and quantitative Methylation specific PCR (MSP)
  • Bisulphite sequencing

OTHER SKILLS

Bioinformatics (12yrs)

Basic R programming Working proficiency on following bioinformatics analysis platforms: Gene expression, Epigenetic, Cancer databases, Transcriptomics, Alternative Splicing.

Program management (10yrs)

Managed projects and teams using TRELLO, SLACK, ASANA).

Statistical Tools (10yrs)

SPSS & MedCalc.

Others

Working proficiency in Microsoft Office, reference managers, WordPress.